Enzyme-linked immunosorbent assay: Difference between revisions
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imported>Robert Badgett (New page: {{subpages}} '''Enzyme-linked immunosorbent assays''' (ELISA) are "an immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either t...) |
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'''Enzyme-linked immunosorbent assays''' (ELISA) are "an [[immunoassay]] utilizing an [[antibody]] labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the [[antigen]] and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed."<ref>{{MeSH}}</ref> | '''Enzyme-linked immunosorbent assays''' (ELISA) are "an [[immunoassay]] utilizing an [[antibody]] labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an [[Immunosorbent technique|immunosorbent]] substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the [[antigen]] and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed."<ref>{{MeSH}}</ref> | ||
==References== | ==References== | ||
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Latest revision as of 03:28, 7 October 2013
Enzyme-linked immunosorbent assays (ELISA) are "an immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed."[1]
References
- ↑ Anonymous (2024), Enzyme-linked immunosorbent assay (English). Medical Subject Headings. U.S. National Library of Medicine.