Patch clamp: Difference between revisions
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Patch-clamping is an electrophysiological recording technique that enables the investigation of single ion channel properties. Using patch clamping it is also possible to assess the electrical properties of small | {{subpages}} | ||
Patch-clamping is an [[Electrophysiology|electrophysiological]] recording technique that enables the investigation of single [[ion]] channel properties. Using patch clamping it is also possible to assess the electrical properties of small [[cell]]s. Recordings are done with glass microelectrodes, similarly as in intracellular recording (although the electrodes are not so sharp). The electrode glass forms an electrically and mechanically tight attachment to the lipids of the cell membrane, so-called giga-seal (referring to the Gigaohm resistance of the junction). | |||
Depending of the configuration of the cell or cell membrane different variants are in use: | Depending of the configuration of the cell or cell membrane different variants are in use: | ||
==cell-attached clamp== | |||
Here the electrode is sealed to the cell membrane allowing investigation of possible ion channel activity on that spot of the membrane. | |||
==whole-cell clamp== | |||
Here the opening of the cell-attached part of the membrane is for voltage-clamping the whole cell or recording its responses in current-clamp mode | |||
==inside-out== | |||
==outside-out== | |||
Here the configurations where the cell patch is pulled from the cell, allows its detailed study. | |||
Invention of patch-clamping is credited to Neher and Sakmann (1976) who got the Nobel prize in | ==History== | ||
Invention of patch-clamping is credited to [[Erwin Neher]] and [[Bert Sakmann]] (1976) who got the Nobel prize in 1991 for this innovation.[[Category:Suggestion Bot Tag]] | |||
Latest revision as of 16:01, 1 October 2024
Patch-clamping is an electrophysiological recording technique that enables the investigation of single ion channel properties. Using patch clamping it is also possible to assess the electrical properties of small cells. Recordings are done with glass microelectrodes, similarly as in intracellular recording (although the electrodes are not so sharp). The electrode glass forms an electrically and mechanically tight attachment to the lipids of the cell membrane, so-called giga-seal (referring to the Gigaohm resistance of the junction).
Depending of the configuration of the cell or cell membrane different variants are in use:
cell-attached clamp
Here the electrode is sealed to the cell membrane allowing investigation of possible ion channel activity on that spot of the membrane.
whole-cell clamp
Here the opening of the cell-attached part of the membrane is for voltage-clamping the whole cell or recording its responses in current-clamp mode
inside-out
outside-out
Here the configurations where the cell patch is pulled from the cell, allows its detailed study.
History
Invention of patch-clamping is credited to Erwin Neher and Bert Sakmann (1976) who got the Nobel prize in 1991 for this innovation.